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phosphatase  (New England Biolabs)
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New England Biolabs phosphatase
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda protein phosphatase  (New England Biolabs)
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New England Biolabs lambda protein phosphatase
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
Lambda Protein Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda phosphatase treatments  (New England Biolabs)
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New England Biolabs lambda phosphatase treatments
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
Lambda Phosphatase Treatments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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λ protein phosphatase  (New England Biolabs)
99
New England Biolabs λ protein phosphatase
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
λ Protein Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+protein+phosphatase+-ppase/Lambda+Protein+Phosphatase/pm42837978-174-11-14
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λ protein phosphatase - by Bioz Stars, 2026-11
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lambda phosphatase  (New England Biolabs)
99
New England Biolabs lambda phosphatase
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
Lambda Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+protein+phosphatase+-ppase/Lambda+Protein+Phosphatase/pm42815464-353-32-34
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lambda pp  (New England Biolabs)
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New England Biolabs lambda pp
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
Lambda Pp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda protein phosphatase new england biolabs  (New England Biolabs)
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New England Biolabs lambda protein phosphatase new england biolabs
(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.
Lambda Protein Phosphatase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+protein+phosphatase+-ppase/Lambda+Protein+Phosphatase/pm42789387-519-16-19
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lambda protein phosphatase new england biolabs - by Bioz Stars, 2026-11
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Image Search Results


(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with Lambda protein phosphatase results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.

Journal: bioRxiv

Article Title: VASH1 phosphorylation regulates tubulin detyrosination via CNP/PRKG1α signaling in DRG neurons

doi: 10.64898/2026.10.14.759141

Figure Lengend Snippet: (A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with Lambda protein phosphatase results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.

Article Snippet: For phosphatase treatment experiments, bead-bound complexes were incubated with phosphatase (Lambda protein phosphatase, New England Biolabs) for 30 min at 30°C according to the manufacturer’s protocol before elution.

Techniques: Confocal, Staining, Control, Western Blot, Cell Culture, Immunoprecipitation, Phospho-proteomics